Vector construction

Obtaining a recombinant plasmid to produce plasmid DNA in large quantities, a recombinant protein or a recombinant vector can be long and fastidious.

SMALTIS can assume the construction of your vectors, whatever the final application.

For that, we deliver a personalized service: sequence design and synthesis, cloning, plasmid preparation, cell line construction and expression control

-Applications for prokaryotic or eukaryotic systems
-Quality control: PCR & Sanger sequencing
-Deliverables: plasmids in solution or cell lines
-Time of construction: 4 weeks

Customized cloning service realised step by step including: :

– Selection and design of the targetted sequence
– Optimization of the sequence according to your specifications
– Selection of DNA synthesis (PCR or gene systhesis)
– Diverse cloning strategies (Restriction, Gateway, SLIC)
– Large choice of plasmids
– Verification of final plasmid by restriction profiles and sequencing

Examples of achievement:

– Construction of bacterial gene-inactivating plasmids
– Construction of an expression plasmid to produce Gag, Pol and Env proteins from HERV virus
– Construction of a Broad-Host range to produce recombinante proteins in Gram negative bacteria