makes microbiology easier

Techniques

The capacities of the laboratory are based on 3 technical platforms

MICROBIOLOGY

› Characterization of microorganisms:
           • identification
           • phenotyping: morphology – physiological & metabolic traits   
           • molecular genotyping
           • antibiotic resistance assessment
           • production of virulence factors measurement 

› Isolation and quantification of bacteria by culture on specific media

› Modification of bacterial genome

MOLECULAR BIOLOGY

›  Nucleic acids preparation

›  Amplification and sequencing of specific sequences by PCR, amplification of circular DNA by Rolling  Circle Amplification (RCA PCR)

›  Vectors construction

CELLULAR BIOLOGY

›  Adhesion & Invasion assays:
           •  various cellular models: intestinal, pulmonary, macrophages, skin cells…
           •  cellular response assessment
           •  quantification by bacerial enumeration and immunolabelling

› Assessment of chemical or bacterial cytotoxicity

› Cellular response to infection: genomic, proteomic