The capacities of the laboratory are based on 3 technical platforms
The microbiology platform has the tools to characterize and quantify microorganisms, whether bacteria, yeast or molds, from different types of samples. This platform also allows us to modify the genome of bacterial strains.
› Characterization of microorganisms:
• phenotyping: morphology – physiological & metabolic traits
• molecular genotyping
• antibiotic resistance assessment
• production of virulence factors measurement
› Isolation and quantification of bacteria by culture on specific media
Whatever the biological sample (blood, cell pellets, microorganisms, biopsies, soils, feces …), Smaltis has the tools to extract the nucleic acids, quantify them by absorbance measurement and verify their integrity. Once prepared, they can be used as a matrix for various applications.
› Amplification, detection and sequencing of genomic fragments by PCR and qPCR, amplification of circular DNA by Rolling Circle Amplification (RCA PCR)
› Metagenomic or metatranscriptomic studies thanks to high throughput sequencing
Thanks to its cell biology platform, Smaltis offers different study models on eukaryotic cells, allowing you to evaluate bacterial infection and to analyze the cellular response to different compounds or microorganisms.
› Adhesion & Invasion assays:
• various cellular models: intestinal, pulmonary, macrophages, skin cells…
• quantification by bacterial enumeration and immunolabelling
› Assessment of chemical or bacterial cytotoxicity
› Cellular response to infection: genomic, proteomic