The capacities of the laboratory are based on 3 technical platforms
MICROBIOLOGY
› Characterization of microorganisms:
• identification
• phenotyping: morphology – physiological & metabolic traits
• molecular genotyping
• antibiotic resistance assessment
• production of virulence factors measurement
› Isolation and quantification of bacteria by culture on specific media
› Modification of bacterial genome
MOLECULAR BIOLOGY
› Nucleic acids preparation
› Amplification and sequencing of specific sequences by PCR, amplification of circular DNA by Rolling Circle Amplification (RCA PCR)
› Vectors construction
CELLULAR BIOLOGY
› Adhesion & Invasion assays:
• various cellular models: intestinal, pulmonary, macrophages, skin cells…
• cellular response assessment
• quantification by bacerial enumeration and immunolabelling
› Assessment of chemical or bacterial cytotoxicity
› Cellular response to infection: genomic, proteomic
