Microbiota: example of achievement

Support of development of EB8018 compound

ISSUE
Demonstrate & validate in vitro the interaction between EB8018 and bacterial protein FimH, a key inducer implied into acute inflammation of patients suffering from Crohn disease.
Create a protocol to isolate from intestinal biopsies the ”biomarkers” bacteria and confirm their interaction with EB8018 compound.

IMPLEMENTATION

ISOLATED STRAINS
Evaluation of adhesion properties of a collection of 110 strains on an intestinal cell-line in the presence or absence of EB8018 by immunolabelling.

Development of an aggregation assay to visualize interaction between EB8018 compound and proteins of strains of interest

BIOPSIES
Develop a method to isolate from intestinal biopsies the ”biomarkers” bacteria.
Confirm the interaction of these “biomarkers” with EB8018 compound.

DELIVERABLES
› Tailor-made operating procedures developed
› Analysis reports and characterized biological samples

ADDED VALUE
› Analysis reports and results compliance with regulatory requirements
› Data security
› Continuation of the project with the end customer