makes microbiology easier

Laboratory

A strategic location

To develop its service activity and R&D projects, SMALTIS was set up, since its creation, in the heart of the TEMIS Health area in Besançon, France. SMALTIS HQ are located in the West part of town in the district known as “Les Hauts du Chazal”, having as principal goal to become a National Center for Biomedical Engineering. Built around the Regional University Hospital Jean Minjoz (CHRU), the French Blood Institution (EFS) of Bourgogne Franche-Comté and the National Institute of Health and Medical Research of Besançon (INSERM), TEMIS Health is a business park specifically designed to offer exceptional setting up conditions to research-oriented enterprises such as SMALTIS.

Two BIOPARC buildings were built with an adjustable 3000 square meter surface area entirely devoted to biomedical activities. Both buildings host innovative companies having micro-technical and multi-technological skills. Among them, let us quote the company Skinexigence, specialized in assessing the efficiency of cosmetic products and with which SMALTIS works to study skin microbiota of volunteers.

It is thus in the BIOPARC building that the founders of SMALTIS have domiciled the head office of the company, on a surface of 250 m² regrouping the office part and the BSL2 laboratory. The latter is divided into three technical platforms on which Smaltis’ services are based.

Two hundred and fifty thousand euros were also invested in the purchase of technical equipment such as an inverted fluorescence microscope, a TECAN Spark 10M microplate reader, type 2 Microbiological Safety stations, a benchtop autoclave, incubators, etc., essential to perform the experiments required to the progress of research programs.

Equipments

MICROBIOLOGY

› 50 m² of BSL2 laboratory
› Anaerobic workstation
› Tecan Microplate Reader
› Electroporator
› Access to a Maldi-Tof (Bruker) mass spectrometer
› Biochemical tests: Gram staining, API gallery
› Thermo-controlled climatic chambers: -80°C/-20°C

MOLECULAR BIOLOGY

› 30 m² of BSL2 laboratory
› Thermal cycler 3 blocks (Biometra)
› Electrophoresis and visualization system
› Dedicated qPCR line
› qPCR devices (Biorad CFX & Qiagen Rotor-gene)
› Dedicated climatic chamber at controlled temperature
› Agilent platform & Nanodrop

CELLULAR BIOLOGY

› 15 m² of BSL2 laboratory
› Inverted fluorescence microscope
› Cell banks stored in liquid nitrogen

Internal capabilities

MICROBIOLOGY

Characterization of microorganisms:

-Identification
-Phenotyping: morphology – physiological & metabolic traits
-Molecular genotyping
-Antibiotic resistance assessment
-Production of virulence factors measurement

> Isolation & Quantification of bacteria by culture on specific media

> Modification of bacterial genome

MOLECULAR BIOLOGY

Amplification of specific sequences by PCR, and of circular DNA by Rolling Circle Amplification (RCA PCR)

Sequencing with qualified providers

Detection of DNA or RNA markers by qPCR

Quantification of gene transcripts by RT-qPCR and assessment of their relative expression

› Expression vectors construction

CELLULAR BIOLOGY

Adhesion & invasion tests:

-Various cellular infection models: intestinal, pulmonary, macrophages, skin cells…

› Assessment of chemical or bacterial cytotoxicity

› Global cellular response:
genomic, proteomic