In vitro evaluation of toxicity

· Determination of Minimal Inhibitory Concentration (MIC) at different pH

  -depending on the way of action of the compound
  -allows to mimic intracellular conditions
· Determination of Lethal Concentration 50 (LC50)

  -concentration able to kill 50% of eukaryotic cells

· Evaluation of hemolytic activity

  -Red Blood Cell Lysis: spectrophotometric measurement of free-hemoglobin
    Red blood cells of different origins (human, sheep, mice…)
    Concentrations close to those used in therapy