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Bacterial Cytotoxicity

The assessment of bacterial cytotoxicity against a cell line consists in measuring the bacterial ability to kill cells.
To evaluate this phenomenon, SMALTIS has developed a method consisting in growing macrophages J774A.1 in presence of your bacteria of interest for a given time and then quantifying cell death.
This indirect quantification is based on the determination of Lactate Dehydrogenase released into the culture medium following cell death.

Sources: ATCC

Example of achievement

     – Construction of a non-cytotoxic mutant derived from Pseudomonas aeruginosa PAO1 strain

Figure: Determination of the percentage of cytotoxicity of the strain PAO1 and its mutant SM46 on the macrophages cell line J774A.1. DMEM F12: negative control; Triton X-100: positive control