The prerequisite for any bacterial infection is the colonization of host tissues. Pathogenic bacteria have thus developed an arsenal of molecules and structures capable of promote their attachment to host cells. This phenomenon, called bacterial adhesion, is an essential step before cell invasion or toxin secretion.
For your needs, SMALTIS has developed different models of cellular infections and different methods to quantify bacterial adhesion and / or invasion.
Models
– Intestinal cells infection models:
. Available Cell lines: Caco2, T84, HT29
. Relevant model to study Enterobacteria (E. coli, K. pneumoniae…)
– Lung cells infection models:
. Available Cell lines: A549 et NCI-H820
. Relevant model to study P. aeruginosa, A. baumannii or S. pneumoniae
– Macrophages infection models:
. Available Cell lines: Murin Macrophages J774A.1
. Relevant model to study phagocytozed bacteria and bacterial cytotoxicity
Other models or cell lines on request.
Methods to quantify
– Counting method
– Staining method with antibodies specific to the studied species
Examples of achievement
– Characterization by immuno-labelling of the adhesion and invasion properties of 100 strains of Escherichia coli on the T84 intestinal line
– Assessment of adhesion/invasion properties of environmental strains (Pantoea sp, Arthobacter sp, Bacillus subtilis)
– Charaterization of mutants derived from Pseudomonas aeruginosa PAO1 strain on A549 and NCI-H820 lung cell lines
– Intracellular activity of antibacterial compounds on S. aureus strains using macrophages J774A.1
– Determination of Lethal Concentration 50 (LC50) of different antibacterial molecules
